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Objective To investigate the effect of leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5 )on the proliferation and drug resistance of HeLa cells and its possible mechanism.Methods LGR5 expression was interfered using shRNA,and LGR5 knockdown HeLa cells were constructed.The effect of LGR5 on the proliferation and drug resistance of HeLa cell was evaluated by cell count,clone formation and MTT;the expressions of LGR5 and β-catenin in HeLa cells were detected by Western blot method.Results LGR5 knockdown HeLa cell line was successfully constructed;the cell growth rate and clone formation rate in shLGR5 group were markedly decreased compared to those in shCon group (P<0 .01 ).Drug resistance of HeLa to cisplatin differed significantly between shLGR5 group and shCon group (P<0.01 ).Moreover,the LGR5 knockdown inhibited the expression ofβ-catenin in HeLa cells.Conclusion LGR5 plays an important role in cell proliferation and drug resistance of HeLa cells,and its mechanism is related to Wnt/β-catenin signaling pathway.
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Objective:To investigate the medical students' cognition of ethical issues in assisted reproductive technology (ART) and discuss the importance of ethical education in medical colleges and universities.Methods:A questionnaire survey about the ethical cognition of assisted reproductive technology was conducted among 632 medical students.The questionnaire included the donation and management of gametes and embryos,and offspring problems.Results:Medical students agreed with most of the current ART-related laws and regulations.however,44.8％ of medical students considered that criteria of donating eggs should be relaxed,such as allowing women who do not receive ART treatment be paid for eggs.In addition,a total of 39.0％ of medical students supported the legalization of surrogacy technology in china.Conclusion:Medical students exists a certain misunderstanding in egg donation and surrogacy technology.We should give more training to medical students in ethical knowledge.In addition,our country can supplement and perfect the current laws and regulations to a certain extent.
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Objective:To investigate the cognitive status of socialist core value among medical students, and to provide reference for the core value education in medical colleges and universities. Methods: 1024 medical students were selected with random cluster sampling method, and the socialist core value was investigated using self-designed questionnaire. Results:It was shown that 69 percent of medical students recognized the importance of socialist core values, but the contents had not been mastered. Social atmosphere, medical student's own short-coming and school education were all the factors leading to the weakness of socialist core values. Conclusion:It is quite necessary to cultivate medical students′socialist core values. Class, school, and society should play the main role to guide the medical students to establish the correct socialist core value.
ABSTRACT
The wavelength of infrared rays,beyond that of red visible light in optical spectre,ranges from 0.76 to 1000.00 μm.In clinic,infrared rays include a near-infrared part(with the wavelength ranging from 0.76 to 1.5μm)and a far-infrared part(with the wavelength ranging from 1.5 to 400μm).The far-infrared rays,having a wavelength ranging from 6 to 14 μm,are critical for human health and lives.The far-infrared rays have many biological functions including extending blood capillary,increasing blood flow,enhancing metabolism and repair,accommodating organism disequilibrium,etc.The main biological effect of far-infrared rays is cell activation which can induce a senes of biochemistry and histochemistry changes of calls.With the help of far-infrared rays,human body can be in a healthy status.The main clinical applications of far-infrared rays include auxiliary diagnosis,softening inflammation and pain.tissue repair,sterilization,tumor depression,rewarming from hypothermia,and so on.Far-infrared rays play active functions on human body through caloric effects.Now they are developed to be applied in partial medical fields.The emphases of the future studies are about the effects of far-infrared rays based on molecular biology researches and about the extensive applications of far-infrared rays in medicine.